Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate
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چکیده
منابع مشابه
Purification and Characterization of Dextransucrase from Leuconostoc Mesenteroides Nrrl B-1149
Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...
متن کاملAcceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase
14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 AB...
متن کاملPreparation and characterization of dextran from Leuconostoc mesenteroides.
In conjunction with investigations on the a-l, 6-glucosidic linkage in starch, it was desirable to conduct comparative studies on other substances containing this linkage. The dextran from Leuconostoc mesenteroides, a polysaccharide having predominantly a-1 ,6-glucosidic linkages, was selected to be used directly in these comparative studies and to provide a source of simpler substances contain...
متن کاملPurification and Characterization of Glucosyltransferases from New Strains Leuconostoc Mesenteroides
The Leuconostoc species require sucrose in the culture medium as an inducer for the elaboration of glucosyltransferase (GTF) and glucans. Among all the reported putification methods, polyethylene glycol (PEG) is a effective and single step purification method for GTF from Leuconostoc mesenteroides. The extracellular GTFs from Leuconostoc mesenteroides L.m.28 and mutant Leuconostoc mesenteroides...
متن کاملCharacterization of the Dextran Produced by Leuconostoc mesenteroides from Date Fruit Extract
In the present study, the production of dextran from date extract and sucrose as carbon sources by the bacterium Leuconostoc mesenteroides NRRL B512 (f) was investigated. In comparison to blue dextran (Mw≈2000 Da), dextran molecular weight was reduced (Mw2000 KDa). Flow behavior indices of dextran solutions from date extracts were investigated and rheological parameters were evaluated at concen...
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ژورنال
عنوان ژورنال: Hemijska industrija
سال: 2017
ISSN: 0367-598X,2217-7426
DOI: 10.2298/hemind160421046m